Where is epo produced in kidneys

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No use, distribution or reproduction is permitted which does not comply with these terms. The growth ratio of cells was examined daily using a Tali image-based cytometer Thermo Fisher Scientific after 1.

Transfection efficiency was estimated by measuring the ratio of GFP-expressing cells in total cells using Tali. The resolved compounds were administered to the cells at a dilution. DMSO or culture medium was used as the vehicle control. The expression levels of housekeeping Hprt hypoxanthine phosphoribosyl transferase mRNA were used as internal standards.

The lysates were transferred to nitrocellulose membranes Bio-Rad after sodium dodecyl sulphate polyacrylamide 8. Cells in culture plates were dissociated with accutase Nakalai Tesque.

Eight clones for each promoter were bilaterally sequenced by using primers Supplementary Table 1 annealing to the cloning site of the pT7Blue T-vector. Subconfluent Replic cells and MEFs in 3. All procedures were performed at room temperature. Subconfluent Replic cells 4. Developed colour intensity was measured using a FilterMax F5 microplate reader Molecular Devices after incubation of the cells with HRP-conjugated streptavidin and fluorescent substrate. All data supporting the results of this study are available in the article and supplementary information files or are available from the authors upon request.

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Am J Physiol Renal Physiol. EPO doping can boost the number of red blood cells and there by increased availability of oxygen to the muscles.

This can help the athlete with increased performance ability and endurance. Blood doping is illegal especially in professional sports. Professional athletes are asked to take this test to find out if they are violating the anti-doping law. About Contact Events News. Search Search. You and Your Hormones. Students Teachers Patients Browse. Human body. Home Hormones Erythropoietin.

Erythropoietin Erythropoietin is a hormone, produced mainly in the kidneys, which stimulates the production and maintenance of red blood cells. Alternative names for erythropoietin Erythropoietin is commonly referred to as EPO. Epo secretion is stimulated by hypoxia, which is detected by an oxygen sensor located in the kidney.

This oxygen sensor has been recently shown to be an heme protein. At the Epo gene level, studies to identify cis-acting DNA sequences, and trans-activation factors for inducible kidney and liver Epo expression are being pursued.